pet-30a(1) vector  (GenScript corporation)

Journal: Marine Drugs

Article Title: Recombinant N-acyl homoserine lactone-Lactonase AiiA QSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp

doi: 10.3390/md17090499

Figure Lengend Snippet: Map of expression plasmid containing aiiA QSI-1 gene.

Article Snippet: The recombinant pET-30a- aiiA QSI-1 vector was then transformed into E. coli BL21(DE3) (Promega, Madison WI,, USA).

Techniques: Expressing, Plasmid Preparation

Journal: Marine Drugs

Article Title: Recombinant N-acyl homoserine lactone-Lactonase AiiA QSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp

doi: 10.3390/md17090499

Figure Lengend Snippet: SDS-PAGE analysis of the recombinant AiiA QSI-1 . M: marker; 1: total protein of noninduced E. coli BL21(DE3)pET30a-aiiA QSI-1 cell lysate; 2: Supernatant of the sonication product of IPTG-induced E. coli BL21(DE3)pET30a-aiiA QSI-1 ; 3: Precipitate of the sonication product of IPTG-induced E. coli BL21(DE3)pET30a-aiiA QSI-1 ; 4: purified AiiA QSI-1 protein (as indicated by arrow).

Article Snippet: The recombinant pET-30a- aiiA QSI-1 vector was then transformed into E. coli BL21(DE3) (Promega, Madison WI,, USA).

Techniques: SDS Page, Recombinant, Marker, Sonication, Purification

Journal: Marine Drugs

Article Title: Recombinant N-acyl homoserine lactone-Lactonase AiiA QSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp

doi: 10.3390/md17090499

Figure Lengend Snippet: AHL-degrading activity bioassays in vivo. Ten μL C6-HSL (10 μM) was mixed with 0 μL ( a ), 20 μL ( b ), 50 μL ( c ), 100 μL ( d ) AiiA QSI-1 , respectively and incubated at 30 °C.

Article Snippet: The recombinant pET-30a- aiiA QSI-1 vector was then transformed into E. coli BL21(DE3) (Promega, Madison WI,, USA).

Techniques: Activity Assay, In Vivo, Incubation

Journal: Marine Drugs

Article Title: Recombinant N-acyl homoserine lactone-Lactonase AiiA QSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp

doi: 10.3390/md17090499

Figure Lengend Snippet: Effect of AiiA QSI-1 on the motility, production of virulence factors and biofilm, and growth of Aerononas hydrophila YJ-1. Swimming motility ( A ), Growth curve of planktonic bacteria ( B ), and biofilm production ( C ).

Article Snippet: The recombinant pET-30a- aiiA QSI-1 vector was then transformed into E. coli BL21(DE3) (Promega, Madison WI,, USA).

Techniques:

Journal: Marine Drugs

Article Title: Recombinant N-acyl homoserine lactone-Lactonase AiiA QSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp

doi: 10.3390/md17090499

Figure Lengend Snippet: Effect of different concentrations of AiiA QSI-1 on A. hydrophila YJ-1 adherence and internalization to EPC. * p < 0.05; ** p < 0.01 in comparison to controls (no added QQ).

Article Snippet: The recombinant pET-30a- aiiA QSI-1 vector was then transformed into E. coli BL21(DE3) (Promega, Madison WI,, USA).

Techniques:

Journal: Marine Drugs

Article Title: Recombinant N-acyl homoserine lactone-Lactonase AiiA QSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp

doi: 10.3390/md17090499

Figure Lengend Snippet: Percent survival of Crucian carp supplement with/without AiiA QSI-1 after Aeromonas hydrophila YJ-1 infection.

Article Snippet: The recombinant pET-30a- aiiA QSI-1 vector was then transformed into E. coli BL21(DE3) (Promega, Madison WI,, USA).

Techniques: Infection